This study was conducted to assess the knowledge, attitude and practice of malaria prevention strategies among mothers of under-five children in North Central Zone, Nigeria. To achieve this purpose, ex-post facto research design was used. A total sample of 768 mothers of under-five children in North-central zone, Nigeria from a population of 3,641,445 were selected through multi-stage sampling procedures of simple random sampling and proportionate sampling. The instrument used for the study was researcher‟s structured questionnaire which was pilot tested using Cronbach Alpha reliability test which was 0.833. Out of the 768 copies of questionnaire distributed, 765(99.6%) were valid for analyses. Inferential statistics of one sample t-test and Pearson Products Moment Correlation Coefficients were used to test the formulated hypotheses at 0.05 level of significance. The results revealed that Knowledge of malaria prevention strategies among mothers of under - five children in north-central zone, Nigeria was significant (p = 0.001), attitude towards malaria prevention strategies among mothers of under-five children in north-central zone, Nigeria was significant (p = 0.000), practice of malaria prevention strategies among mothers of under-five children in north-central zone, Nigeria was not significant (p = 0.7). Based on the results, the following conclusions were drawn, that mothers of under-five children have the knowledge of malaria prevention strategies. Also mothers of under -five children have positive attitude towards malaria prevention strategies in North-central zone of Nigeria. Mothers of under-five children do not practice of malaria prevention strategies in Northcentral zone Nigeria. Based on the conclusions, it was recommended that health educators should carry out awareness campaigns through rendering health talks which would help to further sustain the already existing knowledge of malaria prevention strategies among the mothers of under-five children in North-Central Zone, Nigeria. Health educators in collaboration with other non-governmental and governmental agencies should conduct sensitization campaigns (through mass media or community-based outreach) that would help to further sustain the existing attitudes of mothers of under-five children in North-Central, Nigeria. Health educators should conduct periodic symposia and conferences for mothers of under-five children and women of child-bearing age so as to educate the mothers which would help them to understand the need to apply their knowledge into healthful practices of malaria prevention.