ANALYSIS OF VARIOUS MICROBIAL STAINING TECHNIQUES
By
ILOAMUZOR, SUSSAN C.
Presented To
Department of
Science Laboratory Technology
ABSTRACT
This project work is aimed at carrying out different microbial staining techniques with strict adherence to the field of microbiology. Most bacteria are transparent and so difficult to be seen under the bright field microscope.
Therefore, specimens are routinely showed to increase visibility. Before skinning proper, the organizers are fixed on a slide by either heat fixing method or chemical fixing method. Two mainly types of staining procedure are generally used in skinning as the micro organizer i.e. simple skinning procedure which involves the use of one age and differential staining procedure which employs the use of more than one dye.
The staining techniques analysed include - Tram staining, acid fast staining (ATB), flagella staining. Spare staining. The simple staining procedures reveals this characteristics size and shape of organisms while differential staining help to distinguish between structure within a cell or different types of cells as well as dividing bacteria into gram-positive and gram-negative.
TABLE OF CONTENTS
Title page
Certification
Dedication
Acknowledgement
Abstract
Tale of contents
CHAPTER ONE
INTRODUCTION
1.1 Background information
1.2 Statement of problem
1.3 Aim of study
1.4 Significant of study
1.5 Limitation of study
CHAPTER TWO
2.1 Literature review
2.2 Gram-staining
2.3 Spore staining
2.4 Flagella staining
2.5 Acid-fast staining
CHAPTER THREE
METHODOLOGY
3.1 Gram staining procedures and material
3.2 Acid-fast (afb) materials and procedures
3.3 Spore staining materials and procedures
3.4 Flagella staining materials and procedures
3.5 Unstained wet mount
CHAPTER FOUR
4.1 Unstained bacteria
4.2 Stained bacteria
CHAPTER FIVE
Conclusion and recommendations
5.1 Conclusion
5.2 Recommendation
References
