ABSTRACT
This project is intended to teach and give a general background in culture preparation. It is aimed at giving microbiologist and students specializing in microbiology and related courses the bases on their field of work and study. The preparation of different types of culture media as stipulated in this project, are to guide microbiologist and students in their daily experiences in culturing.
Microorganism as well as acquainting themselves with the techniques and applications involved. These techniques will not only develop the success of the individual, but also guide them to knowing when organisms are present, the type of organism present, then following media mentioned are easy, leap and affordable to prepare for better growth and examination of microorganism. The following are Mac Conkey Agar, which is best to support Gram negative and inhibit Grams positive bacteria.
Another is chocolate (heated blood) agar, it supports the growth of H. influenza, it is also used to culture nutritionally demanding pathogens such as; N. meningitides and finally, blood agar which is gotten from sheep, goat, Horse etc, this contains inhibitions to H. influenza.