This dissertation is on the comparison of health related physical fitness status of male and female basketball and handball players in Borno state, Nigeria. To achieve this purpose, five (5) purposes of the study, five (5) research questions and five (5) hypotheses were used to guide the study. Quasi – experimental research design was used in this study. In the study 102 players are the total population; 80 players were eligible and sampled purposively for this study. The eight, (80) selected by sampling procedure were administered physical fitness test. The test items determined muscular strength, muscular endurance, cardio – respiratory endurance, flexibility, and body comparison. The muscular endurance was determined by the number of sit – ups, the cardio – respiratory endurance was determined by the performance of the subjects in 12munites distance run walk. Flexibility was determined by sit and reach test and muscular strength was determined using standing broad jump. Body composition was determined by sum of skin folds measurement by chest, abdomen, and thigh the descriptive statistics of mean and standard deviation and standard error, and the paired or two sample t – test are used to analyzed the data collected. The findings of this study revealed significant differences between test results of muscular endurance, muscular strength, flexibility, cardio – respiratory endurance and body composition. The study recommended that, physical fitness test should serve as recruitment criteria for players of basketball and handball in any sport competition; also, this study recommended that training programme should be designed to develop more speed and cardio – respiratory endurance for female players in all team sport.