COMPARATIVE ANALYSIS OF ANTIMICROBIAL STRENGTH OF THREE MOST COMMON ANTIBIOTICS USED IN ENUGU

(CASE STUDY OF ANTIBIOTICS DRUGS BROUGHT IN OBIAGU)

By

OSAGIE IZEHIESE BEAUTY

Presented To

Department of Science Technology

ABSTRACT

In comparing the antimicrobial strength of three most common antibiotics, which includes: streptomycin chloranphenicol and gentarccin. 
Samples of urine and high vaginal swab (H.V.S) were collected from park lane Enugu. The organisms isolated were pure culture of staphylococcus aureus and Escherichia coli 
Sensitivity test was carried out with  the use of sensitivity disk containing various minimum inhibitory concentration of the different antibiotics. 
The result obtained shaved that gentamicn was more effective followed by chloramphenicol while some organisms shaved resistant to streptomycin. 






LIST OF TABLE

TABLE 1 NUTRIENT AGAR 
TABLE 2 BLOOD AGAR 
TABLE 3: ANTIBIOTICS AGAINST SYAPHYLOCOCCUS AUREUS 
TABLE 4: ANTIBIOTIC AGAINST ESCHERICHIA COLI 





LIST OF FIGURES

FIGURE 1: STREPTOMYCIN 
FIGURE 2: CHLORAMPHENICOL 
FIGURE3: SENSITIVITY TEST ON STAPHYLOCOCCUS AUREUS 
FIGURE 4 SENSITIVITY TEST ON ESCHERICHIA COLI 
FIGURE 5: STAPHYLOCOCCUS AUREUS AGAINST ANTIBIOTICS 
FIGURE 6: ESCHERICHIA COLI AGAINST ANTIBIOTICS 

TABLE OF CONTENT

Title page
Certification
Dedication
Acknowledgement
Abstract  
List of tables
List of figures
Table of contents
CHAPTER ONE 
1.0 Introduction 
1.1 Historical background 
1.2 Aims and objective 
1.3 Hypothesis 
1.4 Statement of problem 
1.5 Scope of study 
1.6 Limitation of study 

CHAPTER TWO 
2.0 Literature review 
3.1 Procurement of antibiotics 
3.1.1 streptomycin 
2.1.2 Chloramphenicol 
2.1.3 Gentamicin 
2.2 Antimicrobial activity in vitro 
2.3 Antimicrobial activity in vivo 
2.4 Resistance to antimicrobial  drugs 
2.5 Factors affecting antimicrobial activity 
2.6 Clinical use of antibiotics 
2.7 Characteristic of antibiotics 

CHAPTER THREE
3.0 Materials and method 
3.1 Sample collection 
3.1.1 Media composition 
3.1.2 Direction for the preparation of nutrient agar 
3.1.3 Direction for the preparation of blood agar 
3.1.4 Material used for the prewritten of media 

CHAPTER FOUR 
4.0 Isolation and identification procedures 
4.1.1 Isolation and identification of staphylococcus aureus 
4.1.2 Isolation and identification of escherichia coli 
4.2 Sensitivity test 

CHAPTER FIVE 
5.0 RECOMMENDATION 
5.1 CONCLUSION 
REFERENCES



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