ISOLATION AND IDENTIFICATION OF SALMONELLA SPECIES FROM PATIENT SUFFERING FROM TYPHOID FEVER
(A CASE STUDY OF PARK LANE SPECIALIST HOSPITAL, ENUGU)
By
ILOABUSIAKA, CATHERINE T.
Presented To
Department of Science Technology
ABSTRACT
Salmonella Typhi is a rod-shaped Gram-negative bacteria with pesitrichus flagella. They belong to the family Entero bacterioceae and inhabit the large bowel of man. They are found in most food poisoning and tracealole to faecal contaminations. They grow both aerobically and anaerobically and are the main cause of typhoid fever.
Microorganism in the blood sampled from patient of Park Lane Specialist Hospital, Enugu were tested for Salmonella typhi. To do this, Blood samples of 110 patients were collected taking coganizance of their differences in age and sex and using Bacteriological isolation and widal test method. These bloods were cultured and screened appropriately with standard media such as triple sugar iron agar, selenite F. Broth, Deoxychcolate Citrate agar etc.
The result of the two methods were to say the least identical with Nine (9) positive cases displaying active infections and twelve (12) indicating weak infection. While fifty (50) cases tested maintaining could enhance maintaining good sanitary condition, improvement in quality of portable water supply and applicable of proper sewage disposal, could enhance negative prevention of typhoid fever.
TABLE OF CONTENTS
Title page
Certification
Dedication
Acknowledgement
Abstract
Table of Contents
CHAPTER ONE
INTRODUCTION
1.1 Biology of Bacterial Infection (Bacteriology)
1.2 Classification
1.3 Significant of Study
1.4 Statement of Problem
1.5 Objectives of the Study
1.6 Hypothesis
1.7 Limitation of the Study
1.8 Definition of Terms
CHAPTER TWO
LITERATURE REVIEW
2.1 Cultural Characters
2.2 Pathogenicity
2.3 Consequence of Salmonella Typhi-Typhoid fever
2.4 Sources of Infection
2.5 Pathogenesis of Enteric Fever
2.6 Clinical Manifestation
2.7 Laboratory Diagnosis
2.8 Isolation of Salmonella Specie from the Blood
CHAPTER THREE
MATERIALS AND METHODS
3.1 Bacterialogical Isolation of Salmonella Typhi from Blood
3.2 Widal Test
3.3 Agglutination Test
CHAPTER FOUR
RESULT
4.1 Bacteriological Test
4.2 Widal Test Result
4.3 Distribution Table for Patient Tested for Salmonella Typhi (Widal Test)
4.4 Calculation
4.5 Format for Widal Test
CHAPTER FIVE
DISCUSSION, CONCLUSION AND RECOMMENDATION
5.1 Discussion
5.2 Conclusion
5.3 Recommendation
REFERENCES
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