ISOLATION AND IDENTIFICATION OF BACTERIA ASSOCIATED WITH WOUND SEPSIS

By

EZE RUTH ONYINYECHI

Presented To

Department of Science Technology

ABSTRACT

    The isolation and identification of bacteria associated with  150ml sepsis studies were carried out to 250 patient at National Orthopedic Hospital Enugu. (250) Two hundred and fifty patients with 150ml sepstis was grouped in to in patients and out patients.  The  sample collected are wound pus and wound exudates which were collected from patients with diabetic ulcer, surgery wounds, sickle cell ulcers, gun shot wound, using sterile stent striates to  aspirate the wound exudates and steete swab sticks to swab the wound prof. Population was sampled
 According to wound type and noting their differences  sex distributing palternage range (0-2_ years, (3-5_ years (608) years (9-11_ years, (12-14) years, (15-17) years, (18-20) years, and 21 years and above, in sex distribution. (male and female), in predisposing factors and in any other factor affecting it and also their anatibiotic sensitivity pattern.  The samples collected were plated out to on Mac conkey agar, Blood agar, Perory citrate agar (DCA) and further examination was done such as motility test gram staining and some brolhemoceltest like citrate, on and catalyset test foar confirmation.  The results obtained from 250 patients smaples, 199 were positive pathogens and 51 were negative out of which 97 (48.890) were staphylococcus aureus, Staphylococcus aureus was arecorded as the organism that was the highest prevalence while galmorella species has the lowest prevalence 3(1.5%) In this study.  The sensivity patttern of the isolated organisms results show that pseudomonas aeruginosa is resistant to more drugs than the staphylococcus aureus and escherichris coli.  Msot organisms were sensitive ato ciprofloxacillin, Amplicillin, Oxfloxacillin,  Erythroylin, Cotrimoxazole.


TABLE OF CONTENTS

Title page
Certification
Dedication
Acknowledgement
Abstract
Table of contents
List of tables
List of figures

CHAPTER ONE
1.0    Introduction
1.1    complication
1.2    Aim and objectives
1.3    Statement of problems
1.4    The significance of study
1.5    Hypothesis
1.6    Scope of study
1.7    Limitation

CHAPTER TWO
2.0    Literature review
2.1    general incidence to wound sepsis
2.2    Actiological agends of wound sepsis
2.3    Diagnosis and pathogenesis of wound sepsis
2.4    Epideomology of wound sepsis
2.5    Age and sex incidence
2.6    Predisposing factor of wound sepsis
2.7    Chemo therapy of wound sepsis

CHAPTER THREE
3.0    Materials and methods
3.1    materials and culture media used
3.2    Collection and processing sample
3.3    Method
3.4    Biochemical characterization isolates
3.5    Gram staining
3.6    Catalase test
3.7    Coagulase test
3.8    Motilidy test
3.9    Oxidse test
3.10    Indole test
3.11    Methyl red test
3.12    Phenylative deamination test
3.13    Citrase utilization test
3.14    Antibigram

CHAPTER FOUR
4.0    RESULTS

CHAPTER FIVE
5.0    Discussion, suggestions, conclusions and recommendation.
5.1    Discussion
5.2    Suggestion
5.3    Conclusions
5.4    Recommendation

APPENDIX A
REFERENCES



LIST OF TABLES

1.    Subjects smapled accoridng to inpatients, out patients, age and sex distribution
2.    Subjects sampled according to types of wounds, age and sex distribution
3.    Paterns of organisms isolated in wounds sepsis in Enugu
4.    Age and sex distribution of isolates in wound sepsis
5.    Bacterial pathogens causing wound sepsis in patients with sickle cell disease in Enugu
6.    Pattern of bacterial pathogens causing wound sepsis in patients with surgical wounds.
7.    Bacterial isolated in patients with Gunshot wound sepsis
8.    Bacterial isolated from patients with diabetes ulcers
9.    Antibiogram of bacterial isolates from wound sepsis in Enugu
10.    Biochemical reactions and identifications and identification test on bacterial isolates from wound sepsis in Enugu


LIST OF FIGURES

1.    showing organisms isolated from wound sepsis in Enugu (piechart)
2.    Bacterial pathogenesis causing wound sepsis in patients with sickle cell disease
3.    Bacterial pathogens causing wound sepsis in the patients with surgical wounds (Barcharts)
4.    Bacterial isolates from patients with gunshot wound (bar chart)
5.    Bacterial isolates from patients with diabetic ulcer (Bar chart)

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