The study assessed the effects of resistance training on selected biomotor abilities of male football players in Federal College of Education (FCE), Kontagora, Nigeria. Twenty (20) male football players within the age range of 18-29 years participated in the study. They comprised all the playing positions of the football game; that is defence, midfield, forward and goalkeeper. For the purpose of this study, the one (1) group repeated-measures research design was adopted. It provided the organized station of the resistance training around six (6) stations of circuit, consisting of hand dips, lateral pull, leg press, leg curl, leg/knee extension and abdominal curl that were used for the training. Four (4) assessments of participants? biomotor abilities of abdominal strength, VO2 max (endurance), explosive power, flexibility, speed and agility were carried out at base-line, 4th, 8th and 12th week of training respectively. The resistance training programme lasted 12 weeks, on alternate days (Monday, Wednesday and Friday). The data collected were analysed using descriptive and repeated–measures analysis of variance. The findings of the study revealed significant effect of resistance training on abdominal strength (P < 0.001), endurance (P < 0.001), explosive power (P < 0.001), flexibility (P < 0.001), speed (P < 0.001) and agility (P < 0.001) of male football players of FCE Kontagora, Nigeria. Based on the above findings of the study, it was demonstrated, that resistance training programme significantly improved the biomotor abilities of male football players of FCE, Kontagora, Nigeria. The study recommended that biomotor ability training and tests of abdominal strength, endurance (VO2 max), flexibility, explosive power, speed and agility should be carried out regularly by coaches and trainers of male football players of the College. This would improve the general performance of the team and most desirably win titles for the College, especially the seemingly elusive Nigeria Colleges of Education Games Association (NICEGA) Football Championship Cup. Also, the FCE, Kontagora authorities should fully equip or upgrade to acceptable standards, the existing physical fitness laboratory or gym in the College to meet the training needs of all football players. This would further popularize the use of resistance training as a preferred method of improving many physiological variables of football players in the colleges of education and other tertiary institutions in Nigeria. Moreso, Coaches and trainers should be sponsored for further studies at home and abroad to improve their knowledge in modern scientific training methods, especially resistance training method. This would help to establish standards and produce quality football players in colleges of education and other tertiary institution in Nigeria.