The study focused on assessment of availability and utilization of instructional materials for teaching Islamic Studies in junior secondary schools in Kaduna state, Nigeria. It explored, the responses of male and female teachers on relevance of instructional materials; assessed the extent to which urban and rural teachers utilize instructional materials for teaching of Islamic studies and the responses of urban and rural teachers on obstacles to effective utilization of instructional materials in teaching of Islamic Studies in junior secondary schools in Kaduna State. Literature was reviewed in line with the objectives, research questions and hypotheses in the study. Descriptive survey was utilized. Out of the total population of five thousand six hundred and eight-three (5,683) Junior Secondary School teachers in Kaduna State, a sample of one hundred and eighty- three (183) Islamic studies teachers were drawn, through the use of purposive and quota sampling technique. The researcher used instrument titled "Assessment of Availability and Utilization of Instructional Materials for teaching Islamic Studies questionnaire for collecting data. The data collected were descriptively analyzed using mean and standard deviation, while the hypotheses were tested using t-test statistical tool. All the four (4) null hypotheses developed for the study were rejected at 0.05 level of significance. The finding revealed that, Significant difference was found between the responses of male and female teachers on instructional materials available for teaching of Islamic studies in junior secondary schools, Kaduna state; There was no significant difference between the responses of urban and rural teachers on utilization of instructional materials for teaching Islamic Studies in junior secondary schools, Kaduna state; Significant difference did not exist between the responses of urban and rural teachers on relevance of instructional materials in teaching of Islamic Studies in junior secondary schools, Kaduna state; and Significant difference was not found between the responses of male and female teachers on obstacles faced in utilization of instructional materials for teaching of Islamic Studies in junior secondary schools, Kaduna state. The study recommended among others that, the state ministry of education should provide junior secondary schools in Kaduna state with more modern instructional materials such as overhead projectors, film strips, videos and enough computers in order to promote quality teaching and learning in JSS, there is urgent need for government and other stakeholders in education to organise regular workshops/seminars for Islamic Studies teachers and principals on the importance and current developments and progress in the utilization of instructional materials and resources in Islamic studies teaching in the junior secondary schools.