The study was carried out on the impact of millennium development goals retraining programme on job performance and interest among chemistry teachers in daura educational zone, Katsina, Nigeria. It had six objectives, research questions and null hypotheses. Survey research design was used for the study. The population for the study was 79 Chemistry teachers from 36 secondary schools in Daura educational zone which comprises of six Local Governments as sample for the study. These teachers were trained using the Millennium Development Goals retraining programme to form the experimental group, while thirty six (36) teachers who were not exposed to the training formed the control group. The instrument for data collection were adapted version of Egglestone science teachers observation schedule (ETOS), Chemistry Teachers Interest Questionnaire (CTIQ) and Chemistry Performance Test (CPT) for students with reliability coefficients of r=0.67 and r=0.87 respectively were obtained. Mean and standard deviation were used in answering research questions while the PPMC and t-test were used to test the null hypotheses at p≤0.05 level of significance. Null hypotheses (1-5) were rejected while hypothesis six was retained. The study showed that Millennium Development Goals retraining programme had significant influence on students academic performance in chemistry in Katsina State, Nigeria (p=0.000). The Millennium Development Goals training has impacted positively on the job performance of teachers and the academic achievement of their students as well as verifying the level of the teachers. The study recommended the use of Millennium Development Goals retraining programme in training and retraining of chemistry teachers among others.