PHYTOCHEMICAL ANALYSIS OF FLUTED PUMPKIN LEAVE (TELIFAIRIA OCCIDENTALIS)

By

ONYEBUEKE AGATHA .C

Presented To

Department of Science Laboratory Technology

ABSTRACT

Evidence suggests that diet high in vegetable may decrease the risk of chronic disease. Phytochemicals like saponin, flavonoid and tannins from fruits and vegetable may play key roles in amelioration of diseases. Fluted pumpkin leaf (Telfairia Occidentalis) is also a well known vegetable used in the Western part of Nigeria. It is generally called Ugu in Igbo Land. This study was carried out to qualitatively determine the presence of phytochemicals in Telfairia Occidentalis. The widely eaten vegetable in Nigeria. The result of phytochemicals in Telfairia Occidentalis revealed the presence of saponin, tannin alkaloids and slight presence of flavonoids. These results suggest that regular consumption of telfairia Occidentalis could ameliorate malnutrition.

TABLE OF CONTENT

Title page                                                    i
Certification                                                ii
Dedication                                                iii
Acknowledgement                                            iv
Table of content                                            v
Abstract                                                 ix

CHAPTER ONE
INTRODUCTION

1.1    Background of the Study                                1
1.2    Phytochemical Analysis                                2
1.3    Definition of Phytochemicals                            3
1.4    Evidence that Phytochemicals protect against disease        3
1.5    Health benefit of some phytochemicals compound             4
1.6     Aim of the Study                                    5
1.7    Statement of the problem                                5
1.8    Significance of the study                                6
1.9    Limitation of the study                                6

CHAPTER TWO
LITERATURE REVIEW

2.1    Scientific classification of Telfairia Occidentalis                7
2.2    History of pumpkin                                    7
2.3    Health benefit of fluted pumpkin leave                    10
2.4    Growth and development Telfairia Occidentalis                12
2.5    Propagation and planting                                12
2.6    Management                                        13
2.7    Harvesting                                         14
2.8    Handling after harvesting and preservation                 14
2.9    Saponin                                            15
2.10    Alkaloid                                            17
2.11    Flavonoid                                            19

CHAPTER THREE
MATERIAL AND METHOD

3.1    Material                                            20
3.2    Reagent                                            21
3.3    Plant material                                         22
3.4    Sterilization of glass ware                                22
3.5    Preparation of reagent                                23
3.6    Extraction of Technique for fluted pumpkin                23
3.7    Test for alkaloid                                    30
3.8    Test for saponin                                    31
3.9    Test for tannins                                    31
3.10    Test for flavonoid                                     31

CHAPTER FOUR
RESULT                                                33

CHAPTER FIVE
DISCUSSION, CONCLUSION AND RECOMMENDATION
5.1    Discussion                                        34
5.2    Conclusion                                        36
5.3    Recommendation                                    36
    References   

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