EFFECT OF RESVERATROL ON CHLORPYRIFOS-INDUCED COGNITIVE IMPAIRMENT IN SWISS ALBINO MICE

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Department of Medicine

ABSTRACT
Chlorpyrifos (CPF) is thought to cause oxidative injury in both humans and animals via the formation of free radicals. Exposure to CPF at doses that did not result in overt clinical symptoms has been reported among pesticide applicators and other farm workers thereby constituting an important source of occupational hazards to these groups of individuals. Chlorpyrifos (CPF) has been associated with cognitive and psychomotor impairments in both humans and animal. The aim of this work was to evaluate the beneficial role of resveratrol on chlorpyrifos-induced cognitive impairment in Swiss albino mice. Swiss albino mice were divided into (6) six groups of five mice each (n=5). Group I served as the control and were administered olive oil (2 ml/kg), group II received carboxymethylcellulose (CMC) 10g/L, group III received resveratrol 30 mg/kg, group IV received chlorpyrifos (CPF) 3 mg/kg, group V received CPF (3 mg/kg) after the oral administration of resveratrol (30 mg/kg) and group VI received Vitamin E (Vit E) 100 mg/kg. All administrations were done by oral gavage for 21 days. Cognitive function was assessed using Y-maze recognition memory test. And oxidative stress was evaluated using oxidative biomarkers techniques. The results obtained showed that resveratrol at dose 30 mg/kg significantly (p<0.05) improved cognitive impairment. The results showed that resveratrol at dose 30 mg/kg also significantly (p<0.05) improved the activities of superoxide dismutase (SOD) as well as catalase (CAT) when compared with the control. Glutathione (GSH) significantly (p<0.05) increased in the treated group when compared with the control and malondialdehyde (MDA) concentration significantly decreased (p<0.05) when compared with the control. In conclusion, 30mg/kg resveratrol suppressed memory impairment, decreased malondialdehyde levels, increased catalase activity, superoxide dismutase activity and glutathione levels in our chlorpyrifos-induced cognitive impairment mice model.