The study examined the impact of prior exposure to Laboratory apparatus on acquisition of science process skills, academic achievement and retention in chemistry among secondary school students in Giwa Educational Zone. A total of 120 Senior Secondary two (SS II) chemistry students were in the study from a total population of 996. Non randomized pretest, post posttest, posttest experimental - control groups research design was used for the study. Chemistry Achievement Test (CAT) and Science Process Skills Achievement Test (SPSAT)were used as instruments with reliability values of 0.89 and 0.75 respectively. To determine whether students' prior exposed to laboratory apparatus on acquisition of science process skill, academic achievement and retention of different concepts in chemistry, t - test statistical tool at p^0.05 was used to analyse data collected for the study. Generally Prior exposure to laboratory apparatus on acquisition of process skills was found to be more effective in enhancing students academic achievement and retention on chemical concepts than those non prior exposed. The results also showed that both male and female students academic achievement are significantly high given the same condition of exposure to prior laboratory apparatus on acquisition and science- process skills. Therefore, recommendations were made among others that, science curriculum developers, Supervisory bodies of secondary schools, Science educators and teachers should incorporate prior exposure to laboratory apparatus on acquisition of science process skills approach into curricular of chemistry at secondary school level