EVALUATION OF SOME PESTICIDES ON FUSARIUM OXYSPORUM F. SP. STRIGAE, A BIOCONTROL AGENT FOR MANAGING STRIGA HERMONTHICA

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Department of Agricultural Science


ABSTRACT
Five rates of benomyl, carbendazim, mancozeb, hexaconazole, carbendazim + mancozeb, imidacloprid + metalaxyl-M + tebuconazole and metsulfuron methyl were incorporated into molten potato dextrose agar to determine growth and sporulation of PSM 197 and Foxy 2, which were isolates of Fusarium oxysporum f. sp. strigae used against Striga hermonthica. Two hundred and eighty Petri dishes containing amended and un-amended media were inoculated with 14 day-old cultures of PSM 197 and Foxy 2 using flame-sterile 5 mm cork borer. Petri dishes were arranged on laboratory bench in a Completely Randomized Design (CRD) and observations taken at 24 hours interval. Mycelia growth and development of conidia were completely inhibited in media amended with benomyl, carbendazim, and carbendazim + mancozeb. Increasing mancozeb rate from 6 to 24 g/L resulted in lower mycelia diameter and conidia counts for PSM 197 and Foxy 2. Fourteen days after incubation, mycelia diameter of both isolates were low on media amended with highest rates of hexaconazole (1 ml/L), metsulfuron methyl (4.6 g/L) and imidacloprid + metalaxyl-M + tebuconazole (8 g/L). Metsulfuron methyl was adjudged most compatible with the two isolates of Fusarium oxysporum f. sp. strigae on the basis of their mycelial growth, micro-conidial number and conidia germination. With respect to counts macro-conidia, hexaconazole treatments resulted in least variations while viability of conidia was higher with mancozeb treatments followed by metsulfuron methyl. With respect to all parameters considered, imidacloprid + metalaxyl-M + tebuconazole had the highest percent variations than hexaconazole, mancozeb and metsulfuron methyl. The study therefore shows that metsulfuron methyl at 2.3 g/L is compatible with PSM 197 and Foxy 2 and should be used for the management of S. hermonthica.

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