THE DETERMINATION OF THE ACTIVITIES AND SPECIFICITY OF ENZYMES IN FERMENTATION OF STARCH " FROM MAIZE

By

OGIDI DOROTHY A.

Presented To

Department of Science Technology

ABSTRACT

The Anatomy of this project write-up encompasses the ordinary look of enzymes as organic molecules, rather it sheds more light into the comprehensive view and determination of the catalytic and specific activity enzymes particularly- DIASTASE, MALTASE and ZYMASE can exploit in industrial application, also critically important is the selective ways of providing fundamental data which allows the society-particularly small-scale brewers to understand and exploit the chemistry of fermentation by enzymes. The method employed in this project involves three processes namely.
1. The preparation of the enzymes by fermentation
2. The preparation of the starch substrate from maize
3. The overall process development involving both the products of the 1st and 2nd processes. The results of the method and procedures employed in this project are. That enzyme activities in biochemical processes involves catalysis- through binding of the enzyme molecule and the substrates. Finally, all enzymes in fermentation of starch whose catalytic properties rely on their molecular structure and arrangement are stabilized by interactions between their constituents. This is conclusively evident in the chemistry of the fermentation process in starch which is later discussed and highlighted in this write-up.

TABLE OF CONTENTS

Title page
Certification
Dedication
Acknowledgement
Abstract

CHAPTER ONE
Background Information
Aim of the Study
Objectives of the Study
Significance of the Study
Statement of Problem

CHAPTER TWO
Literature Review
Chemistry of Fermentation
The Role of the Enzymes

CHAPTER THREE
Materials and Methodology
Materials and equipments
Methodology and procedures
The Process Development
Preparation of the Enzymes by Fermentation
Preparation of the Starch Substrate from Maize
Flowchart representing the Overall Process Development

CHAPTER FOUR
Results
Conclusion

CHAPTER FIVE
Recommendation
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