TABLE OF CONTENTS
Title page i
Certification page ii
Dedication iii
Acknowledgement iv
Abstract v
Table of contents vi
List of figures x
CHAPTER ONE:
1.0 Introduction 11.1 Background of the study 1
1.2 Statement of problem 4
1.3 Objective of the study 4
1.4 Hypothesis or research questions 5
1.5 Significance of the study 5
CHAPTER TWO:
2.0 Literature Review 62.1 History of Tigernut 6
2.2 Uses of Tigernut 7
2.3 Health benefits of Tigernuts 7
2.4 Nutritional value of Tigernut 11
2.5 Lipids and its effects 12
2.6 Effect of Tigernut on metabolism 14
CHAPTER THREE
3.0 Materials and method 163.1 Material 16
3.2 Chemical Reagents 16
3.3 Methods 17
3.3.1 Collection of sample 17
3.3.2 Extraction of Tigernut oil 17
3.3.3 Care and Management of experimental animal 18
3.3.4 Experimental design 18
3.3.5 Collection of blood samples for Biochemical analysis 20
3.3.6 Determination of cholesterol 20
3.3.7 Determination of triglyceride 22
3.3.8 Determination of HDL cholesterol 24
3.3.9 Determination of LDL cholesterol 26
CHAPTER FOUR:4.0 Results 27
CHAPTER FIVE5.0 Discussion, Conclusion and Recommendation 33
5.1 Discussion 33
5.1.1 Effect of tigernut extract on serum cholesterol of rats 33
5.1.2 Effect of Tigernut extract on serum triglyceride of rats 34
5.1.3 Effect of Tigernut extract on serum HDL of rats 34
5.1.4 Effects of Tigernut on serum LDL of rats 34
5.1.5 Effect of Tigernut extract on body weight of rats 35
5.2 Conclusion 35
5.3 Recommendation 36
References 7
Appendix 40
LIST OF FIGURESfigure 1: Serum cholesterol concentration 28
figure II: Serum triglyceride concentration 29
figure III: Serum HDL-C concentration 30
figure iv: Serum LDL-C concentration 31
figure v: Percentage increase in body weight 32